Tao T W, Jenkins J M, Vosbeck K, Matter A, Miller M, Jockusch B M, Shen Z H, Burger M M
Int J Cancer. 1983 Feb 15;31(2):239-47. doi: 10.1002/ijc.2910310218.
Lectin-resistant variants of B-16 melanoma cells were selected with wheat-germ agglutinin, ricin and concanavalin A. They exhibited altered metastasizing capacity and tumorigenicity in C57BL mice. Several in vitro properties were defined and compared including homotypic adhesiveness, microfilament organization, melanin release, activity of tyrosine hydroxylase, DNA content, and karyotypes. The possible relevance of these properties in vitro for the malignant behavior displayed in vivo is discussed. The usefulness of this approach of selecting surface variants to study the problem of metastasis is also discussed.
用小麦胚芽凝集素、蓖麻毒素和伴刀豆球蛋白A筛选出了对凝集素具有抗性的B - 16黑色素瘤细胞变体。它们在C57BL小鼠中表现出转移能力和致瘤性的改变。定义并比较了几种体外特性，包括同型黏附性、微丝组织、黑色素释放、酪氨酸羟化酶活性、DNA含量和核型。讨论了这些体外特性与体内显示的恶性行为的可能相关性。还讨论了这种选择表面变体来研究转移问题的方法的实用性。
