Fung S J, Boxer P A, Morales F R, Chase M H
Brain Res. 1982 Sep 30;248(2):267-73. doi: 10.1016/0006-8993(82)90584-4.
Intracellular recordings were obtained from lumbar motoneurons in unanesthetized, undrugged, normally respiring cats during the states of wakefulness, quiet sleep and active sleep. The objective was to examine the state-dependent control of spinal cord motoneurons exerted by the pontomesencephalic reticular formation. Accordingly, electrical stimulation was applied to the nucleus reticularis pontis oralis while the membrane potential of lumbar motoneurons was recorded. Short latency depolarizing and/or hyperpolarizing potentials were observed throughout sleep and wakefulness; no state-dependent pattern was found in the direction of polarization or amplitude for these early potentials. However, a long latency hyperpolarizing potential emerged exclusively during active sleep; it was characterized by a peak latency of 45 +/- 1 (S.E.M.) ms, a duration of 40 +/- 2 ms, and an amplitude of 3 +/- 0.5 mV. This active sleep-selective potential was capable of inhibiting spontaneous motoneuron activity. These and previously obtained data support the notion that excitation of the nucleus reticularis pontis oralis results in somatomotor inhibition at the level of the spinal cord and brainstem selectively during the state of active sleep.
在未麻醉、未用药、正常呼吸的猫处于清醒、安静睡眠和活跃睡眠状态时，从腰段运动神经元获取细胞内记录。目的是研究脑桥中脑网状结构对脊髓运动神经元的状态依赖性控制。因此，在记录腰段运动神经元膜电位的同时，对脑桥嘴侧网状核施加电刺激。在整个睡眠和清醒过程中均观察到短潜伏期去极化和/或超极化电位；这些早期电位的极化方向或幅度未发现与状态相关的模式。然而，一种长潜伏期超极化电位仅在活跃睡眠期间出现；其特征为峰值潜伏期45±1（标准误）毫秒，持续时间40±2毫秒，幅度3±0.5毫伏。这种活跃睡眠选择性电位能够抑制运动神经元的自发活动。这些以及先前获得的数据支持这样一种观点，即脑桥嘴侧网状核的兴奋在活跃睡眠状态下选择性地导致脊髓和脑干水平的躯体运动抑制。
