Green S A, Jenkins S J, Clark P A
Cornell Vet. 1982 Oct;72(4):416-26.
The biuret total protein method and a bromcresol green (BCG) albumin method were used on the Abbott ABA-100 chemistry analyzer to assay serum proteins in clinically normal cattle, sheep, ponies, pigs, and ducks. Total proteins were also read on a refractometer and mylar supported cellulose acetate electrophoresis was performed. Globulins and A/G ratios were calculated from the chemical method and the results compared with the electrophoretic method. Total protein, albumin and A/G ratios in the ponies, sheep and older cattle were in agreement between the two methods. The younger cattle and all the pigs had higher albumin levels and A/G ratios with the chemical BCG method. Ducks had slightly higher albumin values and A/G ratios with the electrophoretic method and the presence of pre-albumin was detected. Typical mylar supported cellulose acetate electrophoretic patterns are presented which show the excellent separation using these membranes. Means and range for normal animals are given and changes of proteins with age are discussed.
采用双缩脲总蛋白法和溴甲酚绿（BCG）白蛋白法，在雅培ABA - 100化学分析仪上对临床正常的牛、羊、小马、猪和鸭的血清蛋白进行检测。同时使用折射仪读取总蛋白，并进行聚酯薄膜支持的醋酸纤维素电泳。根据化学方法计算球蛋白和A/G比值，并将结果与电泳法进行比较。小马、绵羊和成年牛的总蛋白、白蛋白和A/G比值在两种方法之间一致。幼年牛和所有猪的化学BCG法检测的白蛋白水平和A/G比值更高。鸭的电泳法检测的白蛋白值和A/G比值略高，并且检测到前白蛋白的存在。给出了典型的聚酯薄膜支持的醋酸纤维素电泳图谱，显示了使用这些膜的出色分离效果。给出了正常动物的均值和范围，并讨论了蛋白质随年龄的变化。
