Everett J W, Tyrey L
Endocrinology. 1982 Dec;111(6):1979-85. doi: 10.1210/endo-111-6-1979.
Adult proestrous rats were subjected to either electrochemical or electrical stimulation of the medial preoptic area after ovulation-blocking dosage with either pentobarbital (PTBL), morphine, chlorpromazine, or atropine. For electrochemical stimulation (ECS), 233 microA anodal DC for 30 sec (7000 mu coulombs) was delivered through a unipolar stainless steel electrode. For electrical stimulation (ES), 750 microA biphasic pulse pairs at 30 Hz, on and off each 15 sec, were delivered through a coaxial platinum electrode, in four 5-min bursts equally spaced during 60 min. In PTBL-blocked rats this stimulus produced LH surges equivalent to those after continuous stimulation for 60 min. Blood was collected 60 and 90 min after ECS or after the start of ES. Mean serum LH concentrations (RIA) maximal at 90 min after ECS, were similar under the four blocking agents (1270-1486 ng/ml serum in terms of NIAMDD LH RP-1). Like-wise, after ES there were no significant differences (P greater than 0.05) among the mean LH levels at 60 min (310-571 ng/ml serum). The 90-min values showed a downward, but not significant, trend in the case of PTBL, chlorpromazine, and atropine plus PTBL. Under morphine an apparent upward trend was due largely to an animal having an especially large increase. With rare exception, full ovulation was evident at terminal laparotomy on the morning after stimulation. Thus, whichever of the several drugs was used, medical preoptic area stimulation with given parameters induced section of comparable amounts of LH. None of the drugs appears to have primary suppressive action on the preoptic-tuberal system, on the availability of LHRH or its release into the portal vessels, on the ability of the pituitary to release LH, or on the ovarian response.
成年动情前期大鼠在接受戊巴比妥（PTBL）、吗啡、氯丙嗪或阿托品排卵阻断剂量处理后，对内侧视前区进行电化学或电刺激。对于电化学刺激（ECS），通过单极不锈钢电极施加233微安阳极直流电，持续30秒（7000微库仑）。对于电刺激（ES），通过同轴铂电极以30赫兹的频率施加750微安双相脉冲对，每15秒开启和关闭一次，在60分钟内等间隔分成四个5分钟的脉冲串。在PTBL阻断的大鼠中，这种刺激产生的促黄体生成素（LH）峰与持续刺激60分钟后的LH峰相当。在ECS后或ES开始后60分钟和90分钟采集血液。ECS后90分钟时血清LH浓度平均值（放射免疫分析法）达到峰值，在四种阻断剂作用下相似（以NIAMDD LH RP - 1计，血清中为1270 - 1486纳克/毫升）。同样，ES后60分钟时平均LH水平（310 - 571纳克/毫升血清）之间无显著差异（P大于0.05）。在PTBL、氯丙嗪和阿托品加PTBL的情况下，90分钟的值呈下降趋势，但不显著。在吗啡作用下，明显的上升趋势主要归因于一只动物的特别大幅增加。除极少数例外，在刺激后次日早晨的终末剖腹手术中可见完全排卵。因此，无论使用几种药物中的哪一种，以给定参数刺激视前区均诱导分泌相当量的LH。这些药物似乎均未对视前 - 结节系统、促性腺激素释放激素（LHRH）的可用性或其向门静脉血管的释放、垂体释放LH的能力或卵巢反应产生主要抑制作用。
