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Radiochemical assay for gamma-aminolevulinate synthase.

作者信息

Brooker J D, Srivastava G, May B K, Elliott W H

出版信息

Enzyme. 1982;28(2-3):109-19. doi: 10.1159/000459095.

Abstract

We describe here a standard protocol for the radiochemical assay of gamma-aminolevulinate synthase from 17-day chick embryo liver mitochondria and starved male rat liver mitochondria. The assay is sensitive to at least 15 pmol of the gamma-aminolevulinate produced and can be adapted to suit most tissue types. A number of possible modifications to the procedure are discussed.

摘要

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