兔耳动脉去神经和去中枢节后对钾离子超敏反应的比较研究

Comparative study of supersensitivity to K+ after denervation and decentralization in rabbit ear artery.

作者信息

Tsuru H, Bevan R D, Shigei T

出版信息

Eur J Pharmacol. 1982 Oct 15;84(1-2):9-16. doi: 10.1016/0014-2999(82)90151-0.

Abstract

The contractile response to potassium following denervation or decentralization of the central artery of the rabbit ear was studied in vitro. Denervation or decentralization for 8 weeks caused comparable supersensitivity to potassium: mean EC50 values obtained with isotonic high potassium solution were 21.3 mM in the denervated artery and 18.7 mM in the decentralized artery, and these values were significantly less than those (25-26 mM) of their respective contralateral control arteries (P less than 0.005, paired t-test). The maximum responses to potassium did not differ among the control, denervated and decentralized arteries. Phentolamine (3 X 10(-7) M) and propranolol (3 X 10(-7) M) had no significant effect on the response to potassium. Sensitivities to potassium with hypertonic high KCl solution and hypertonic high K2SO4 solution were virtually identical to those obtained with isotonic high potassium solution. The results suggest that denervation and decentralization produce identical postjunctional supersensitivity to potassium and that the lack of tonic activity of sympathetic nerves may be responsible for the phenomenon in the rabbit ear artery.

摘要

在体外研究了兔耳中央动脉去神经或中枢神经离断后对钾的收缩反应。去神经或中枢神经离断8周会导致对钾产生类似的超敏反应：用等渗高钾溶液测得的平均半数有效浓度（EC50）值，去神经动脉为21.3 mM，中枢神经离断动脉为18.7 mM，这些值显著低于各自对侧对照动脉的值（25 - 26 mM）（配对t检验，P < 0.005）。对照、去神经和中枢神经离断动脉对钾的最大反应没有差异。酚妥拉明（3×10⁻⁷ M）和普萘洛尔（3×10⁻⁷ M）对钾的反应没有显著影响。用高渗高氯化钾溶液和高渗高硫酸钾溶液时对钾的敏感性与用等渗高钾溶液时几乎相同。结果表明，去神经和中枢神经离断对钾产生相同的节后超敏反应，并且交感神经紧张性活动的缺乏可能是兔耳动脉中该现象的原因。