George H A, Smibert R M
J Bacteriol. 1982 Dec;152(3):1060-5. doi: 10.1128/jb.152.3.1060-1065.1982.
Spectrophotometric assays of pyruvate oxidation catalyzed by extracts of the Reiter strain of Treponema phagedenis indicated that viologen dyes, flavin nucleotides, and a ferric iron chelate, but not pyridine nucleotides, were utilized as electron acceptors. Benzyl viologen-linked activity partially sedimented during ultracentrifugation and appeared similar to clostridial pyruvate:ferredoxin oxidoreductase with respect to the spectral properties of the enzyme chromophore. Electron carrier activity in treponemal extracts was quantitated by a metronidazole-linked assay in which the oxidation of pyruvate by carrier-depleted extracts led to the reduction of electron carrier in the crude extracts which then reduced metronidazole. The rate of metronidazole reduction was proportional to the amount of electron carrier present in the assay. Electron carrier activity in Triton X-100-solubilized, crude extracts partially purified by DEAE-cellulose chromatography and gel filtration was attributed to a protein possessing the spectral and physical properties of a ferredoxin. A similar protein appeared to be present in extracts of Treponema denticola ST10.
噬菌密螺旋体赖特尔菌株提取物催化丙酮酸氧化的分光光度法测定表明，紫精染料、黄素核苷酸和一种铁螯合物（而非吡啶核苷酸）被用作电子受体。在超速离心过程中，苄基紫精连接的活性部分沉淀，就酶发色团的光谱特性而言，其与梭菌丙酮酸：铁氧化还原蛋白氧化还原酶相似。通过甲硝唑连接的测定法定量梅毒螺旋体提取物中的电子载体活性，其中载体耗尽的提取物氧化丙酮酸导致粗提取物中的电子载体还原，然后该电子载体还原甲硝唑。甲硝唑还原速率与测定中存在的电子载体量成正比。经DEAE - 纤维素色谱和凝胶过滤部分纯化的Triton X - 100增溶粗提取物中的电子载体活性归因于一种具有铁氧化还原蛋白光谱和物理性质的蛋白质。在齿垢密螺旋体ST10提取物中似乎也存在类似的蛋白质。
