Murase T, Yamada N, Akanuma Y, Ohsawa N
J Biochem. 1982 Sep;92(3):817-21. doi: 10.1093/oxfordjournals.jbchem.a133994.
Triacylglycerol lipase activities in adrenals and testes of rats were measured and the properties of the enzymes were characterized. The enzymes were extracted from acetone-ether dried powder of the tissues and the crude extracts were applied to a heparin-Sepharose affinity column. The enzymes bound to the column were then eluted at different ionic strengths. Two lipases could be detected in rat adrenals: one was inactivated by the antiserum raised against hepatic triacylglycerol lipase and the other had the properties of lipoprotein lipase. In testes, only lipoprotein lipase could be detected. The results are discussed in connection with the possible functions of these two lipases in organs utilizing lipoprotein-cholesterol for steroid hormone synthesis.
测定了大鼠肾上腺和睾丸中三酰甘油脂肪酶的活性，并对这些酶的性质进行了表征。从组织的丙酮 - 乙醚干粉中提取酶，将粗提取物应用于肝素 - 琼脂糖亲和柱。然后在不同离子强度下洗脱结合在柱上的酶。在大鼠肾上腺中可检测到两种脂肪酶：一种被针对肝脏三酰甘油脂肪酶产生的抗血清灭活，另一种具有脂蛋白脂肪酶的性质。在睾丸中，仅能检测到脂蛋白脂肪酶。结合这两种脂肪酶在利用脂蛋白胆固醇进行类固醇激素合成的器官中的可能功能对结果进行了讨论。
