Kikuchi S, Kusaka T
J Biochem. 1982 Sep;92(3):839-44. doi: 10.1093/oxfordjournals.jbchem.a133997.
Beside de novo fatty acid synthetase, two kinds of fatty acid elongating systems have been found in Mycobacterium smegmatis; they are the malonyl-CoA-dependent, acyl carrier protein (ACP)-requiring system (fatty acid synthetase II, named by K. Bloch's group, 1969) and the acetyl-CoA-dependent ACP-non-requiring one (acetyl-CoA-dependent elongation of fatty acids reported by us, 1977). Now, a third fatty acid elongating system, malonyl-CoA-dependent and ACP-non-requiring, has been isolated from an extract of this microorganisms, separately from each of the previous two elongating systems. Primer specificity and cofactor requirements, especially of pyridine-nucleotide-coenzyme, of the last elongation system also distinguish it from the two previously known systems. All three systems, however, were found in a soluble fraction of M. smegmatis, therefore the regulatory mechanism for these elongation systems should be investigated hereafter.
除了从头脂肪酸合成酶外，在耻垢分枝杆菌中还发现了两种脂肪酸延长系统；它们是依赖丙二酰辅酶A、需要酰基载体蛋白（ACP）的系统（脂肪酸合成酶II，由K.布洛赫小组于1969年命名）和依赖乙酰辅酶A、不需要ACP的系统（我们于1977年报道的脂肪酸的乙酰辅酶A依赖性延长）。现在，已经从这种微生物的提取物中分离出第三种脂肪酸延长系统，该系统依赖丙二酰辅酶A且不需要ACP，与前两种延长系统分开。最后一种延长系统的引物特异性和辅因子需求，尤其是吡啶核苷酸辅酶的需求，也使其与之前已知的两种系统有所区别。然而，所有这三种系统都存在于耻垢分枝杆菌的可溶部分中，因此，这些延长系统的调控机制有待进一步研究。
