一种与胃肠道癌相关的单克隆抗体定义抗原是一种含有唾液酸化乳糖-N-岩藻五糖II的神经节苷脂。

A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II.

作者信息

Magnani J L, Nilsson B, Brockhaus M, Zopf D, Steplewski Z, Koprowski H, Ginsburg V

出版信息

J Biol Chem. 1982 Dec 10;257(23):14365-9.

Abstract

Two monoclonal antibodies produced by hybridomas obtained from a mouse immunized with a colorectal carcinoma cell line bind specifically to human gastrointestinal cancer cells. The antigen of this antibody in the carcinoma cell line, a monosialoganglioside, has been isolated. Its carbohydrate structure is probably (formula: see text). This oligosaccharide is a sialyl derivative of lacto-N-fucopentaose II, a hapten of the human Lea blood group antigen. About 30 micrograms of ganglioside is obtained from 1 g of cells, wet weight. The ganglioside was detected by autoradiography in lipid extracts of some carcinomas and of meconium, but not in lipid extracts of normal adult tissues. Antigen was detected by solid phase radioimmunoassay in lipid extracts from 12 out of 21 adenocarcinomas of the colon, from 4 out of 5 gastric adenocarcinomas, and from 4 out of 7 pancreatic carcinomas. Antigen was not detected in lipid extracts from 5 esophageal carcinomas or from normal colon and gastric mucosa, pancreas, kidney, liver, and bone marrow.

摘要

用结肠癌细胞系免疫小鼠获得的杂交瘤产生的两种单克隆抗体可特异性结合人胃肠道癌细胞。该癌细胞系中此抗体的抗原——一种单唾液酸神经节苷脂已被分离出来。其碳水化合物结构可能为（分子式：见正文）。这种寡糖是乳糖 - N - 岩藻五糖II的唾液酸衍生物，而乳糖 - N - 岩藻五糖II是人类Lea血型抗原的一种半抗原。每克湿重细胞可获得约30微克神经节苷脂。通过放射自显影在一些癌组织和胎粪的脂质提取物中检测到了神经节苷脂，但在正常成人组织的脂质提取物中未检测到。通过固相放射免疫测定法在21例结肠腺癌中的12例、5例胃腺癌中的4例以及7例胰腺癌中的4例的脂质提取物中检测到了抗原。在5例食管癌以及正常结肠、胃黏膜、胰腺、肾脏、肝脏和骨髓的脂质提取物中未检测到抗原。