单唾液酸神经节苷脂是一种结肠癌的单克隆抗体定义抗原。
A monosialoganglioside is a monoclonal antibody-defined antigen of colon carcinoma.
作者信息
Magnani J L, Brockhaus M, Smith D F, Ginsburg V, Blaszczyk M, Mitchell K F, Steplewski Z, Koprowski H
出版信息
Science. 1981 Apr 3;212(4490):55-6. doi: 10.1126/science.7209516.
PMID:7209516
Abstract
The antigen of a monoclonal antibody that is specific for cells of human carcinoma of the colon is a monosialoganglioside as determined by the direct binding of antibody to thin-layer chromatograms of total lipid extracts of tissues. Binding of antibody to chromatograms is detected by autoradiography after the application of iodine-125-labeled F(ab')2 of rabbit immunoglobulin G antibodies to mouse immunoglobulins.
摘要
通过抗体与组织总脂质提取物的薄层色谱直接结合确定，一种对人结肠癌细胞具有特异性的单克隆抗体的抗原是一种单唾液酸神经节苷脂。在用碘-125标记的兔免疫球蛋白G抗体对小鼠免疫球蛋白的F(ab')2应用于色谱图后，通过放射自显影检测抗体与色谱图的结合。
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