Nilsson O, Månsson J E, Lindholm L, Holmgren J, Svennerholm L
FEBS Lett. 1985 Mar 25;182(2):398-402. doi: 10.1016/0014-5793(85)80341-0.
A novel ganglioside was detected in a small cell lung carcinoma by TLC-immunostaining of gangliosides with a monoclonal antibody, the C-50 MAb. Structural characterization showed this ganglioside to be IV3NeuAc-LcOse4Cer, a hitherto unknown ganglioside. This ganglioside has also been detected as a minor component in many different carcinomas using the C-50 MAb. The normally dominant CA-50 ganglioside antigen is IV3NeuAc, III4Fuc-LcOse4Cer. Based upon solid-phase binding to IV3NeuAc, III4-LcOse4Cer and IV3NeuAc-LcOse4Cer it is concluded that the C-50 MAb recognizes an epitope present in sialylated type I carbohydrate chains.
通过用单克隆抗体C-50 MAb对神经节苷脂进行薄层色谱免疫染色，在小细胞肺癌中检测到一种新型神经节苷脂。结构表征表明这种神经节苷脂是IV3NeuAc-LcOse4Cer，一种迄今未知的神经节苷脂。使用C-50 MAb在许多不同的癌组织中也检测到这种神经节苷脂作为次要成分。正常占主导地位的CA-50神经节苷脂抗原是IV3NeuAc、III4Fuc-LcOse4Cer。基于与IV3NeuAc、III4-LcOse4Cer和IV3NeuAc-LcOse4Cer的固相结合，得出结论：C-50 MAb识别唾液酸化I型碳水化合物链中存在的一个表位。
