Schwartz K, Lompre A M, Bouveret P, Wisnewsky C, Whalen R G
J Biol Chem. 1982 Dec 10;257(23):14412-8.
Rat cardiac ventricular myosins were obtained from fetuses, from young normal animals, and from hypophysectomized adults. The purified proteins were compared by several techniques: (i) electrophoresis in non-denaturing conditions (pyrophosphate buffer), (ii) one- and two-dimensional analysis after proteolytic cleavage, (iii) immunological blotting after electrophoretic purification, and (iv) competitive enzyme-linked immunosorbent assay. Antibodies specific to each of the two major isoenzymes of adult rat heart (V1 and V3 according to the terminology of Hoh et al. (Hoh, J. F. Y., McGrath, P. A., and Hale, P. T. (1978) J. Mol. Cell. Cardiol. 10, 1053-1076) were used for the immunological studies. The heavy chains of the ventricular myosin isoenzymes of fetuses (V3F) were indistinguishable from those of the V3H isoenzyme present in hypophysectomized adults; both proteins differed from the V1 isoform of young animals. The light chains of V3F, V3H, and V1 were the same, except that V3F contained in addition a small amount of the embryonic light chain (Whalen, R. G., and Sell, S. M. (1980) Nature 286, 731-733). These results strongly suggest that adaptation of the adult rat heart to the hormonal deficiencies of hypophysectomy is mediated by the synthesis of the same myosin heavy chain form which is predominant in fetal hearts.
大鼠心室肌球蛋白取自胎儿、年轻正常动物以及垂体切除的成年动物。通过几种技术对纯化后的蛋白质进行比较：（i）在非变性条件下（焦磷酸缓冲液）进行电泳；（ii）蛋白水解切割后的一维及二维分析；（iii）电泳纯化后的免疫印迹；（iv）竞争性酶联免疫吸附测定。针对成年大鼠心脏两种主要同工酶（根据霍等人的术语，即V1和V3（Hoh, J. F. Y., McGrath, P. A., and Hale, P. T. (1978) J. Mol. Cell. Cardiol. 10, 1053 - 1076））的特异性抗体用于免疫研究。胎儿心室肌球蛋白同工酶（V3F）的重链与垂体切除成年动物中存在的V3H同工酶的重链无法区分；这两种蛋白质均与幼龄动物的V1同工型不同。V3F、V3H和V1的轻链相同，只是V3F还含有少量胚胎轻链（Whalen, R. G., and Sell, S. M. (1980) Nature 286, 731 - 733）。这些结果有力地表明，成年大鼠心脏对垂体切除所致激素缺乏的适应性是由合成在胎儿心脏中占主导的相同肌球蛋白重链形式介导的。
