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酿酒酵母细胞质和线粒体核糖体中的免疫相关蛋白。

Immunologically related proteins in cytoplasmic and mitochondrial ribosomes of yeast Saccharomyces cerevisiae.

作者信息

Sudarickov A B, Surguchov A P

出版信息

Mol Gen Genet. 1986 May;203(2):316-9. doi: 10.1007/BF00333973.

DOI:10.1007/BF00333973
PMID:3526092
Abstract

Ribosomal proteins from the cytoplasm and mitochondria of the yeast Saccharomyces cerevisiae were compared by immunoblotting techniques. Antibodies raised against cytoplasmic ribosomal proteins cross-react with five mitochondrial ribosomal proteins, four of which are located in the large and one in the small mitochondrial subunits. The possible existence of common ribosomal proteins for cytoplasmic and mitochondrial ribosomes is discussed.

摘要

利用免疫印迹技术比较了酿酒酵母细胞质和线粒体中的核糖体蛋白。针对细胞质核糖体蛋白产生的抗体与五种线粒体核糖体蛋白发生交叉反应,其中四种位于线粒体大亚基,一种位于小亚基。文中讨论了细胞质核糖体和线粒体核糖体可能存在共同核糖体蛋白的情况。

相似文献

1
Immunologically related proteins in cytoplasmic and mitochondrial ribosomes of yeast Saccharomyces cerevisiae.酿酒酵母细胞质和线粒体核糖体中的免疫相关蛋白。
Mol Gen Genet. 1986 May;203(2):316-9. doi: 10.1007/BF00333973.
2
Comparison of amino acid compositions of mitochondrial and cytoplasmic ribosomal proteins of Saccharomyces cerevisiae.酿酒酵母线粒体和细胞质核糖体蛋白的氨基酸组成比较。
Mol Gen Genet. 1979 Mar 27;171(3):335-41. doi: 10.1007/BF00267589.
3
Protein composition of Saccharomyces cerevisiae mitochondrial ribosomes.酿酒酵母线粒体核糖体的蛋白质组成。
Acta Biochim Pol. 1988;35(2):105-18.
4
P5/P5' the acidic ribosomal phosphoproteins from Saccharomyces cerevisiae.
Biochim Biophys Acta. 1980;606(1):76-82. doi: 10.1016/0005-2787(80)90099-4.
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Cytoplasmic ribosomal proteins from Chlamydomonas reinhardtii: characterization and immunological comparisons.莱茵衣藻的细胞质核糖体蛋白:特性及免疫学比较
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6
Characterization of a novel acidic protein of 38 kDa, A0, in yeast ribosomes which immunologically cross-reacts with the 13 kDa acidic ribosomal proteins, A1/A2.
J Biochem. 1987 Dec;102(6):1565-70. doi: 10.1093/oxfordjournals.jbchem.a122205.
7
Protein composition of the bovine mitochondrial ribosome.牛线粒体核糖体的蛋白质组成。
J Biol Chem. 1982 Aug 10;257(15):8788-94.
8
Acidic proteins from Saccharomyces cerevisiae ribosomes.
Biochem Biophys Res Commun. 1979 Mar 15;87(1):281-91. doi: 10.1016/0006-291x(79)91677-2.
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A study of mitochondrial ribosomes from the higher plant Solanum tuberosum L.
Mol Biol Rep. 1986;11(2):93-7. doi: 10.1007/BF00364820.
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The ribosomal proteins of Saccharomyces cerevisiae.酿酒酵母的核糖体蛋白。
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本文引用的文献

1
Cloning and identification of a DNA fragment coding for the sup1 gene of Saccharomyces cerevisiae.克隆和鉴定编码酿酒酵母 sup1 基因的 DNA 片段。
Curr Genet. 1984 Aug;8(6):467-70. doi: 10.1007/BF00433913.
2
Core particles and proteins from mitochondrial ribosomes of yeast.酵母线粒体核糖体的核心颗粒与蛋白质
FEBS Lett. 1971 Jun 24;15(3):186-190. doi: 10.1016/0014-5793(71)80308-3.
3
Experiences using chloramine-T and 1, 3, 4, 6-tetrachloro-3 alpha, 6 alpha-diphenylglycoluril (Iodogen) for radioiodination of materials for radioimmunoassay.
使用氯胺 - T和1,3,4,6 - 四氯 - 3α,6α - 二苯基甘脲(碘甘脲)对放射免疫分析材料进行放射性碘化的经验。
J Clin Chem Clin Biochem. 1981 Oct;19(10):1051-6.
4
Comparisons of rat cardiac myosins at fetal stages in young animals and in hypothyroid adults.对幼龄动物胎儿期、成年甲状腺功能减退大鼠的心肌肌球蛋白进行比较。
J Biol Chem. 1982 Dec 10;257(23):14412-8.
5
Defects in modification of cytoplasmic and mitochondrial transfer RNAs are caused by single nuclear mutations.细胞质和线粒体转运RNA修饰缺陷由单核基因突变引起。
Cell. 1982 Mar;28(3):543-50. doi: 10.1016/0092-8674(82)90209-4.
6
Isopentenylation of both cytoplasmic and mitochondrial tRNA is affected by a single nuclear mutation.细胞质和线粒体tRNA的异戊烯基化受到单个核突变的影响。
J Biol Chem. 1982 Sep 25;257(18):10562-5.
7
Ribosomal recessive suppressors cause a respiratory deficiency in yeast Saccharomyces cerevisiae.核糖体隐性抑制子会导致酿酒酵母出现呼吸缺陷。
Mol Gen Genet. 1982;185(2):319-23. doi: 10.1007/BF00330805.
8
Relationship between cytoplasmic and mitochondrial apparatus of protein synthesis in yeast Saccharomyces cerevisiae.酿酒酵母中蛋白质合成的细胞质和线粒体机制之间的关系。
Mol Gen Genet. 1983;189(1):172-4. doi: 10.1007/BF00326073.
9
"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.“蛋白质免疫印迹法”:蛋白质从十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳转移至未修饰的硝酸纤维素膜上,并用抗体和放射性碘化蛋白A进行放射自显影检测。
Anal Biochem. 1981 Apr;112(2):195-203. doi: 10.1016/0003-2697(81)90281-5.
10
Phenylalanyl-tRNA synthetases from yeast cytoplasm and mitochondria. The presence of a carbohydrate moiety in the mitochondrial enzyme and immunological evidence for structural relationship.
Biochim Biophys Acta. 1983 Mar 30;743(3):451-4. doi: 10.1016/0167-4838(83)90405-3.