Goldstein M E, Sternberger N H, Sternberger L A
J Neuroimmunol. 1982 Nov;3(3):203-17. doi: 10.1016/0165-5728(82)90023-6.
Adult and developing rat cerebella were stained immunocytochemically with six neuron-specific monoclonal antibodies obtained from spleen cells of BALB/c mice immunized with hypothalamus. Monoclonal peroxidase-antiperoxidase complex was used in the staining procedure. In the adult, three different staining patterns were seen with these antibodies. The general patterns have been designated as (1) neurofibrillar, (2) perikaryal-neurofribillar, and (3) synapse-associated. In addition, each antibody within a designated group showed a different immunocytochemical distribution. Some antibodies reacted widely, for example, with many neuronal perikarya and fibers, and their distribution increased with the development of the brain. Other antibodies had a more restricted distribution and stained only some structures within an area or pathway. To account for this type of restriction of distribution, we propose that there may be microheterogeneity of some of the antigens visualized and have called this microheterogeneity 'neurotypy'. A second type of restriction was also observed. With several antibodies a loss of staining occurred in the adult cerebellum in structures that had reacted during early development. These differences in staining probably reflect developmental regulation of the antigens (neurotypes).
用从经下丘脑免疫的BALB/c小鼠脾细胞中获得的六种神经元特异性单克隆抗体，对成年和发育中的大鼠小脑进行免疫细胞化学染色。染色过程中使用单克隆过氧化物酶-抗过氧化物酶复合物。在成年大鼠中，用这些抗体观察到三种不同的染色模式。一般模式已被指定为(1)神经原纤维型、(2)核周-神经原纤维型和(3)突触相关型。此外，指定组内的每种抗体都显示出不同的免疫细胞化学分布。一些抗体反应广泛，例如，与许多神经元核周体和纤维反应，并且它们的分布随着大脑的发育而增加。其他抗体的分布更局限，仅对一个区域或通路内某些结构染色。为了解释这种分布限制类型，我们提出，所显示的某些抗原可能存在微异质性，并将这种微异质性称为“神经型”。还观察到第二种限制类型。使用几种抗体时，在成年小脑中，早期发育期间有反应的结构出现染色缺失。这些染色差异可能反映了抗原(神经型)的发育调控。
