Aizenman Y, Weichsel M E, de Vellis J
Proc Natl Acad Sci U S A. 1986 Apr;83(7):2263-6. doi: 10.1073/pnas.83.7.2263.
A pure neuronal culture grown in a defined serum-free environment has been developed and characterized. Insulin was the only hormone found to enhance the growth of neurons obtained from embryonic chicken brains during the early proliferative stage, a time when many neurons survived without the addition of any growth factors to the culture. With increasing embryonic age, there was an increase in the number of neurons requiring transferrin. By the time neurons reached a postmitotic state in older brains, they were completely dependent on both insulin and transferrin for survival and growth. Because this culture is free of glial cells and serum, it provides an effective basis for investigating molecular mechanisms underlying neuronal development.
一种在特定无血清环境中培养的纯神经元培养物已被开发并表征。胰岛素是唯一被发现能在早期增殖阶段促进从胚胎鸡脑中获得的神经元生长的激素，在这个阶段，许多神经元在培养物中不添加任何生长因子的情况下也能存活。随着胚胎年龄的增加，需要转铁蛋白的神经元数量增加。当神经元在较老的大脑中达到有丝分裂后状态时，它们的存活和生长完全依赖于胰岛素和转铁蛋白。由于这种培养物不含神经胶质细胞和血清，它为研究神经元发育的分子机制提供了一个有效的基础。
