Särnstrand B, Brattsand R, Malmström A
J Invest Dermatol. 1982 Dec;79(6):412-7. doi: 10.1111/1523-1747.ep12530360.
Human skin fibroblasts were exposed to 3 anti-inflammatory steroids in order to study their effects on the glycosaminoglycan metabolism. The potent glucocorticoids, fluocinolone acetonide and budesonide, even at low concentrations strongly reduced the accumulation of hyaluronic acid and sulfated glycosaminoglycans in the medium, at the cell surface, and in the cells. Hydrocortisone had considerably less effect. The 3 compartments were not influenced to the same extent and the least inhibition was noted in the cell surface pool. Dermatan sulfate was decreased to the same relative extent in all 3 compartments, while hyaluronic acid and heparan sulfate were specifically retained at the cell surface, explaining why this compartment was less affected than the others. Dermatan sulfate was studied in more detail regarding effects on its copolymeric structure. Glucocorticoid treatment changed the uronosyl composition of the polysaccharides so that a relative decrease of glucuronic acid residues and a relative increase of iduronic acid residues were noted. This change was most evident in dermatan sulfate of the medium and of the cell surface. Thus, glucocorticoid treatment not only reduces the quantity of various glycosaminoglycans but also changes the distribution, the relative proportion, and the structure of connective tissue proteoglycans. These effects probably contribute to the development of skin atrophy, which often is observed after long-term treatment with potent glucocorticoids.
为研究三种抗炎类固醇对糖胺聚糖代谢的影响，将其作用于人类皮肤成纤维细胞。强效糖皮质激素醋酸氟轻松和布地奈德，即使在低浓度时也能显著减少培养基、细胞表面和细胞内透明质酸和硫酸化糖胺聚糖的积累。氢化可的松的作用则小得多。这三个区室受到的影响程度不同，细胞表面池受到的抑制作用最小。硫酸皮肤素在所有三个区室中相对减少程度相同，而透明质酸和硫酸乙酰肝素则特异性地保留在细胞表面，这就解释了为什么这个区室比其他区室受影响小。对硫酸皮肤素对其共聚结构的影响进行了更详细的研究。糖皮质激素处理改变了多糖的糖醛酸组成，使得葡萄糖醛酸残基相对减少，艾杜糖醛酸残基相对增加。这种变化在培养基和细胞表面的硫酸皮肤素中最为明显。因此，糖皮质激素处理不仅减少了各种糖胺聚糖的数量，还改变了结缔组织蛋白聚糖的分布、相对比例和结构。这些作用可能促成了皮肤萎缩的发生，长期使用强效糖皮质激素后常常会出现这种情况。
