来自紫色链霉菌SANK 95570的pSV1质粒对亚甲基新生霉素A合成的测定

Determination of methylenomycin A synthesis by the pSV1 plasmid from Streptomyces violaceus-ruber SANK 95570.

作者信息

Aguilar A, Hopwood D A

出版信息

J Gen Microbiol. 1982 Aug;128(8):1893-901. doi: 10.1099/00221287-128-8-1893.

Abstract

A plasmid (pSV1) of 110 x 10(6) daltons from a methylenomycin A producing strain of Streptomyces violaceus-ruber was detected on an isolated from agarose gels. Elimination of this plasmid by protoplasting and regeneration resulted in the simultaneous loss of methylenomycin A production and resistance. pSV1 hybridized with pBR322 containing a cloned fragment of 1.7 x 10(6) daltons from S. coelicolor A3(2) which codes for methylenomycin A resistance. The pSV1 plasmid could be transferred to S. lividans by conjugation and by transformation and plasmid DNA identical in size to pSV1 could be isolated from the recipient strains. These experiments show that pSV1 codes for methylenomycin A production and resistance, in close analogy to the SCP1 plasmid from S. coelicolor A3(2).

摘要

从紫色红链霉菌的美亚霉素A产生菌株中分离出一个110×10⁶道尔顿的质粒（pSV1），它是在琼脂糖凝胶上检测到的。通过原生质体形成和再生消除该质粒，导致美亚霉素A产量和抗性同时丧失。pSV1与含有来自天蓝色链霉菌A3(2)的1.7×10⁶道尔顿克隆片段的pBR322杂交，该片段编码美亚霉素A抗性。pSV1质粒可通过接合转移到淡紫灰链霉菌，也可通过转化转移，并且可以从受体菌株中分离出大小与pSV1相同的质粒DNA。这些实验表明，pSV1编码美亚霉素A的产生和抗性，这与来自天蓝色链霉菌A3(2)的SCP1质粒非常相似。