Stonesifer J, Matsushima P, Baltz R H
Mol Gen Genet. 1986 Mar;202(3):348-55. doi: 10.1007/BF00333261.
Genes encoding enzymes for tylosin biosynthesis, genes involved in the expression of resistance to tylosin (Tyl), hygromycin B (Hm), chloramphenicol (Cm), and mitomycin C (MC), and a single copy of an amplifiable unit of DNA (AUD) were jointly transferred at very high frequencies by conjugation from several different Streptomyces fradiae strains to S. fradiae JS85, a mutant defective in many or possibly all tylosin biosynthetic reactions and containing a multiple tandem reiteration of the AUD. No recombination was observed between nar, rif and spc genes in conjugal matings, but recombination was observed between these genes after protoplast fusion. Tylosin biosynthetic genes were transferred at a much lower frequency to S. fradiae JS87, another mutant defective in many or all tylosin biosynthetic reactions, but deleted for the AUD and other DNA sequences. These findings suggest that tylosin structural genes, several genes encoding antibiotic resistance determinants, and amplifiable DNA are present on a self-transmissible element that does not mobilize chromosomal genes, and that JS85 and JS87 contain deletions, and JS85 an amplification, of overlapping portions of this element.
编码泰乐菌素生物合成酶的基因、与对泰乐菌素（Tyl）、潮霉素B（Hm）、氯霉素（Cm）和丝裂霉素C（MC）的抗性表达相关的基因，以及一个可扩增DNA单元（AUD）的单拷贝，通过接合作用以非常高的频率从几种不同的弗氏链霉菌菌株共同转移到弗氏链霉菌JS85中，JS85是一种在许多或可能所有泰乐菌素生物合成反应中存在缺陷的突变体，并且含有AUD的多个串联重复序列。在接合交配中，未观察到nar、rif和spc基因之间发生重组，但在原生质体融合后观察到这些基因之间发生了重组。泰乐菌素生物合成基因以低得多的频率转移到弗氏链霉菌JS87中，JS87是另一种在许多或所有泰乐菌素生物合成反应中存在缺陷的突变体，但缺失了AUD和其他DNA序列。这些发现表明，泰乐菌素结构基因、几个编码抗生素抗性决定簇的基因和可扩增DNA存在于一个不移动染色体基因的自我传递元件上，并且JS85和JS87含有该元件重叠部分的缺失，JS85还含有该元件的扩增。
