Serwer P, Masker W E, Allen J L
J Virol. 1983 Feb;45(2):665-71. doi: 10.1128/JVI.45.2.665-671.1983.
Attempts were made to increase the efficiency of infectious particle formation during the in vitro assembly of bacteriophage T7 from procapsids and DNA. It was found that dextrans and some smaller, related compounds (sucrose and sorbitol) increase this efficiency by a factor of 8 to 50. Dextrans also inhibited elevated temperature-induced emptying of DNA from bacteriophages T7, P22, and T4, suggesting that the stimulation of assembly is caused, at least in part, by the stabilization of packaged DNA in capsids. The data indicated that the sugars and polyols can slow DNA emptying from bacteriophages at elevated temperature whether they permeate the bacteriophage capsid or not. In contrast, the data suggested that permeation of some particle, probably a capsid, results in inhibition of in vitro T7 assembly.
人们尝试在体外由原衣壳和DNA组装噬菌体T7的过程中提高感染性颗粒形成的效率。结果发现，葡聚糖以及一些较小的相关化合物（蔗糖和山梨醇）可将该效率提高8至50倍。葡聚糖还抑制了高温诱导的噬菌体T7、P22和T4中DNA的排空，这表明组装的刺激作用至少部分是由衣壳中包装DNA的稳定化引起的。数据表明，无论糖类和多元醇是否渗透到噬菌体衣壳中，它们都能在高温下减缓噬菌体中DNA的排空。相比之下，数据表明某些颗粒（可能是衣壳）的渗透会导致体外T7组装受到抑制。
