Salat-Baroux J, Scemama H, Agnes E, Couturier J Y, Lestrat N, Mandelbaum J, Plachot M, Firmin C, Feinstein-Soldate H C, Fiet J, Vuillard E, Cornet D, Boccara U
Nouv Presse Med. 1982 Sep 18;11(36):2681-5.
The results of 36 follicular punctures for in vitro fertilization (IVF) performed at the Maternity unit of Hôpital Tenon, Paris, between January and June 1981 are reported. To collect a pre-ovulatory ovocyte by follicular puncture through laparoscopy requires extremely accurate monitoring of ovulation to make sure that the ovocyte collected has completed its nuclear and cytoplasmic maturation in vivo. The criteria for puncture time are the beginning of LH plasma peak level in spontaneous cycles and injection of HCG in stimulated cycles. In this series the ovocyte collection rate was 60% on average. Failures were mostly due to the local status of the genital tract in the women selected for attempted IVF, since all of them had permanent tubal sterility consecutive to severe pelvic inflammatory lesions. The percentage of ovocytes found to be mature was higher in spontaneous than in stimulated cycles. Out of 13 pre-ovulatory ovocytes collected, 8 were fertilized in vitro. Four could be transferred into the uterus, resulting in two pregnancies which had to be interrupted at an early stage. IVF demands full availability of the surgical and laboratory teams concerned.
报告了1981年1月至6月在巴黎特农医院妇产科进行的36次体外受精（IVF）卵泡穿刺结果。通过腹腔镜卵泡穿刺采集排卵前卵母细胞需要极其精确地监测排卵情况，以确保采集到的卵母细胞在体内已完成核成熟和胞质成熟。穿刺时间的标准是自然周期中促黄体生成素（LH）血浆峰值水平开始时，以及促排卵周期中注射人绒毛膜促性腺激素（HCG）时。在该系列研究中，卵母细胞采集率平均为60%。失败主要是由于选择进行体外受精尝试的女性生殖道局部状况，因为她们均因严重盆腔炎性病变而导致永久性输卵管不育。发现成熟卵母细胞的百分比在自然周期中高于促排卵周期。在采集的13个排卵前卵母细胞中，有8个在体外受精。4个可移植到子宫内，结果有两次妊娠，但不得不早期终止。体外受精需要相关手术和实验室团队全员到位。
