Gorokhov A F, Pupkova V I
Prikl Biokhim Mikrobiol. 1982 Sep-Oct;18(5):725-9.
This paper describes a modification of the method of direct elution of substances from chromatographic plates and the method based on preliminary concentration on a small area of the sorbent and its subsequent extraction. These methods can be used for simultaneous extraction of several samples from chromatographic plates on a flexible support in 30-50 microliter of the solvent. The paper discusses the selection of solvent systems to be applied for substance extraction from plates. It also considers advantages and disadvantages of the methods as illustrated by nucleic acid elution from the plates Silufol UF-254 and amino acid elution from the plates Fixion 50X8. The elution of the components reaches 97-99%. The process can be performed in routine laboratory glassware.
本文描述了对从色谱板直接洗脱物质的方法以及基于在吸附剂小面积上进行预浓缩及其后续萃取的方法的改进。这些方法可用于在30 - 50微升溶剂中从柔性载体上的色谱板同时萃取多个样品。本文讨论了用于从板上萃取物质的溶剂系统的选择。还通过从Silufol UF - 254板上洗脱核酸以及从Fixion 50X8板上洗脱氨基酸来说明这些方法的优缺点。各组分的洗脱率达到97 - 99%。该过程可在常规实验室玻璃器皿中进行。
