Etingov E D, Baevskiĭ D Iu, Slavin A A, Iunkerova L I, Savina N V
Antibiotiki. 1978 Aug;23(8):686-9.
A thin-layer chromatographic method for separation of novobiocin, isonovobiocin and descarbamylnovobiocin using Silufol plates was developed. The method is simple and rapid. It provided clear separation of the components and using of diethyl ether, a simple individual separating solvent. The method allowed determination of novobiocin and the products of its isomerization in the fermentation broth, extracts and dry substances, as well as performance of operative control and regulation of the technological processes of fermentation and chemical purification of novobiocin.
开发了一种使用硅胶板分离新生霉素、异新生霉素和去甲氨甲酰基新生霉素的薄层色谱法。该方法简单快速。它能清晰分离各组分，且使用乙醚这种简单的单一分离溶剂。该方法可用于测定发酵液、提取物和干物质中的新生霉素及其异构化产物，还可用于新生霉素发酵和化学纯化工艺过程的操作控制与调节。
