Palacios E, Osada J, Arce C, Aylagas H, Santos-Ruiz A
Rev Esp Fisiol. 1982;38 Suppl:135-40.
The effect of thioacetamide on rat liver phospholipids biosynthesis was investigated using 32P as precursor. The incorporation of 32P-ortophosphate in the polar lipid fraction and the specific radioactivities of individual phospholipids in the liver fraction and the specific radioactivities of individual phospholipids in the liver of control rats and rats treated with thioacetamide were determined 75 min after intraperitoneal administration of 32P-ortophosphate. Intraperitoneal injection of thioacetamide (daily dose of 100 mg/kg body weight) in male Wistar rats resulted in an increase of 32P incorporation in the over-all phospholipids, after 8 doses administration and a significant decrease during the chronic intoxication. Specific activity of phosphatidylcholine decreased and a parellel increase in specific radioactivity of lisophosphatidylcholine was found after three days of thioacetamide treatment. There was also observed a marked increase in specific radioactivity of sphingomyelin, which could be due to a stimulation of CDP-choline: ceramide cholinephosphotransferase reaction with subsequent diminution of the rate of phosphatidylcholine synthesis via the CDP-amine pathway (involving cytidine diphosphocholine).
以32P作为前体，研究了硫代乙酰胺对大鼠肝脏磷脂生物合成的影响。腹腔注射32P-正磷酸盐75分钟后，测定对照大鼠和硫代乙酰胺处理大鼠肝脏部分中极性脂质部分的32P-正磷酸盐掺入量、各磷脂的比放射性以及肝脏中各磷脂的比放射性。雄性Wistar大鼠腹腔注射硫代乙酰胺（每日剂量100mg/kg体重），在给药8次后，总体磷脂中的32P掺入量增加，而在慢性中毒期间显著降低。硫代乙酰胺处理三天后，磷脂酰胆碱的比活性降低，溶血磷脂酰胆碱的比放射性平行增加。还观察到鞘磷脂的比放射性显著增加，这可能是由于CDP-胆碱：神经酰胺胆碱磷酸转移酶反应受到刺激，随后通过CDP-胺途径（涉及胞苷二磷酸胆碱）的磷脂酰胆碱合成速率降低所致。
