Abraham M, Hilge V, Lison S, Tibika H
Cell Tissue Res. 1984;235(2):403-10. doi: 10.1007/BF00217866.
Structural and functional relationships between oocytes and their envelopes were studied by means of electron microscopy in several teleost species after injection of live fish with horseradish peroxidase. The marker first appeared in the capillaries and the pericapillary spaces of the ovarian stroma. It then entered the collagen-filled spaces between the granulosa and theca cells; these spaces are in direct connection with the pericapillary spaces. The marker penetrated between the follicle cells and into the channels of the zona radiata surrounding the microvilli which traverse these channels. The marker was never found inside the microvilli or in the follicle cells; finally, it reached the surface of the oocytes and was internalized via micropinocytosis. Six stages in the course of folliculogenesis were observed, determined by (1) the formation of follicular and thecal cellular layers and a collagen-filled space between them, (2) the development of microvilli of oocytal and follicular origin, (3) the differentiation of the vitelline envelope and the pore channels, (4) pinocytotic activity of the oocytes, and (5) rapid growth of the oocyte and its envelopes during vitellogenesis.
在用辣根过氧化物酶注射活鱼后，通过电子显微镜研究了几种硬骨鱼卵母细胞与其包膜之间的结构和功能关系。标记物首先出现在卵巢基质的毛细血管和毛细血管周围间隙中。然后它进入颗粒细胞和卵泡膜细胞之间充满胶原的间隙；这些间隙与毛细血管周围间隙直接相连。标记物穿透卵泡细胞之间并进入围绕穿过这些通道的微绒毛的放射带通道。标记物从未在微绒毛内部或卵泡细胞中发现；最后，它到达卵母细胞表面并通过微胞饮作用被内化。观察到卵泡发生过程中的六个阶段，其由以下因素决定：(1) 卵泡和卵泡膜细胞层的形成以及它们之间充满胶原的间隙；(2) 卵母细胞和卵泡来源的微绒毛的发育；(3) 卵黄膜和孔道的分化；(4) 卵母细胞的胞饮活性；(5) 卵黄发生期间卵母细胞及其包膜的快速生长。
