Davis N T
Stain Technol. 1982 Jul;57(4):239-44. doi: 10.3109/10520298209066714.
A Parafilm disk floating on saline and bearing a drop of cobalt solution in which the nerve stump is bathed provides a convenient and versatile method for in vitro or in vivo filling of neurons for cobalt sulfide staining. Silicone grease around the edge of the disk provides an effective seal around the nerve as it passes between the two solutions. Using a modified developer, silver intensification of cobalt sulfide stained neurons may be done in the light at room temperature, and the time of optimum intensification may be observed under a dissecting microscope.
将一片Parafilm膜漂浮在盐溶液上，并在上面滴一滴钴溶液，使神经残端浸泡其中，这为硫化钴染色的神经元进行体外或体内填充提供了一种方便且通用的方法。圆盘边缘的硅脂在神经穿过两种溶液之间时能在其周围形成有效的密封。使用改良显影剂，硫化钴染色的神经元的银强化可在室温光照下进行，并且可以在解剖显微镜下观察到最佳强化时间。
