Hofreiter B T, Allen J P, Mizera A C, Powers C D, Masi A M
Steroids. 1982 May;39(5):547-55. doi: 10.1016/0039-128x(82)90055-1.
Problems inherent in corticosterone radioimmunoassay (RIA) led to consideration of alternative methods. A high-performance liquid chromatography (HPLC) procedure was evaluated that separated and quantitated dichloromethane-extracted corticosterone by reverse-phase chromatography. The results were correlated (r = 0.92) with an RIA procedure. The HPLC recovered nearly 100% of corticosterone added to rat plasma and had excellent reproducibility. In addition, chromatogram profiles of dichloromethane-soluble components obtained from rat plasma, derived from drug effect studies, could have value for characterizing response patterns. Without automated sample injection equipment, HPLC is more appropriately applied in monitoring RIA results than in processing large numbers of samples.
皮质酮放射免疫测定法（RIA）固有的问题促使人们考虑采用其他方法。对一种高效液相色谱（HPLC）方法进行了评估，该方法通过反相色谱法分离并定量二氯甲烷提取的皮质酮。结果与放射免疫测定法相关（r = 0.92）。高效液相色谱法从添加到大鼠血浆中的皮质酮中回收率接近100%，且具有出色的重现性。此外，从药物效应研究获得的大鼠血浆中可溶于二氯甲烷的成分的色谱图，对于表征反应模式可能具有价值。若没有自动进样设备，高效液相色谱法更适合用于监测放射免疫测定结果，而非处理大量样本。
