Beazell J W, Adomian G E, Furmanski M, Tan K S
Am Heart J. 1982 Dec;104(6):1328-34. doi: 10.1016/0002-8703(82)90163-6.
Methods for producing complete heart block in experimental animals have usually required thoracotomy to gain access to the intracardiac conduction system. A closed chest technique has been developed for producing discrete permanent lesions in the atrioventricular node or His bundle of anesthetized dogs. An insulated transseptal needle is passed through a venous catheter to the right atrium. The exposed tip is pressed into the His bundle and a 30 to 40 joule pulse from a DC defibrillator is transmitted through the needle. This produces a 1 to 3 mm diameter coagulative lesion which permanently destroys the tissue at this point. The process can be repeated until complete heart block is achieved. Our procedure has been successful in 45 of 46 attempts to prepare dogs for acute experiments and, using implanted pacemakers, for chronic studies. We conclude that this closed chest method is a safe and reliable alternative for production of complete heart block requiring thoracotomy, thereby providing an important new means for both acute and chronic experimental studies requiring complete heart block for the improved elucidation of mechanisms and management of cardiovascular diseases.
在实验动物中产生完全性心脏传导阻滞的方法通常需要开胸以进入心脏内传导系统。现已开发出一种闭合胸腔技术，用于在麻醉犬的房室结或希氏束中制造离散的永久性损伤。一根绝缘的经中隔针通过静脉导管插入右心房。将暴露的针尖压入希氏束，并通过该针传输来自直流除颤器的30至40焦耳脉冲。这会产生一个直径为1至3毫米的凝固性损伤，在此处永久性地破坏组织。该过程可重复进行，直到实现完全性心脏传导阻滞。我们的方法在46次为急性实验准备犬以及使用植入式起搏器进行慢性研究的尝试中，有45次取得了成功。我们得出结论，这种闭合胸腔方法是一种安全可靠的替代方法，可用于替代需要开胸的完全性心脏传导阻滞的产生，从而为需要完全性心脏传导阻滞以更好地阐明心血管疾病机制和治疗方法的急性和慢性实验研究提供了一种重要的新手段。
