人及公猪精子膜泡的分离与特性分析：利用氮气空化和离子载体诱导膜泡形成的方法

Isolation and characterization of membrane vesicles from human and boar spermatozoa: methods using nitrogen cavitation and ionophore induced vesiculation.

作者信息

Gillis G, Peterson R, Russell L, Hook L, Freund M

出版信息

Prep Biochem. 1978;8(5):363-78. doi: 10.1080/10826067809412288.

Abstract

A method for isolation of plasma membrane vesicles from human and boar spermatozoa using nitrogen cavitation is described. The purity of the preparations were assessed by electron microscopy, marker enzyme assay and the sedimentation characteristics of fused plasma membrane-acrosomal membrane vesicles in sucrose gradients. PAGE-SDS profiles of plasma membrane polypeptides from boar spermatozoa were significantly different from those of human spermatozoa. Differences in electrophoretic profiles of polypeptides from different regions of the spermatozooon were also observed.

摘要

本文描述了一种使用氮气空化从人及公猪精子中分离质膜囊泡的方法。通过电子显微镜、标记酶测定以及蔗糖梯度中融合质膜 - 顶体膜囊泡的沉降特性来评估制备物的纯度。公猪精子质膜多肽的SDS - PAGE图谱与人精子的显著不同。还观察到精子不同区域多肽电泳图谱的差异。