公猪精子亚细胞组分的一种新分离方法。

A new separation method of subcellular fractions of boar spermatozoa.

作者信息

Töpfer-Petersen E, Schill W B

出版信息

Andrologia. 1981 Mar-Apr;13(2):174-6. doi: 10.1111/j.1439-0272.1981.tb00027.x.

DOI:10.1111/j.1439-0272.1981.tb00027.x

Abstract

A new procedure for the isolation of acrosomal membranes of boar spermatozoa by centrifugation in modified colloidal silica gradients is described. The carbohydrate composition of the membrane fractions is reported. The acrosomal membrane fraction (Mf 1) contains about 10% carbohydrates which are composed of fucose, mannose, galactose, N-acetylglucosamine, N-acetylgalactosamine and N-acetylneuraminic acid in the molar ratio 1:3:6:4.5:2:3.

摘要

本文描述了一种通过在改良的硅胶梯度中离心分离公猪精子顶体膜的新方法。报告了膜组分的碳水化合物组成。顶体膜组分（Mf 1）含有约10%的碳水化合物，其由岩藻糖、甘露糖、半乳糖、N-乙酰葡糖胺、N-乙酰半乳糖胺和N-乙酰神经氨酸以1:3:6:4.5:2:3的摩尔比组成。