DiBona G F, Sawin L L
Am J Physiol. 1982 Dec;243(6):F576-80. doi: 10.1152/ajprenal.1982.243.6.F576.
To assess the effect of renal nerve stimulation on sodium, chloride, and water transport in the loop of Henle, experiments were performed in anesthetized hydropenic and isotonic saline volume-expanded rats using renal clearance and Henle's loop microperfusion techniques (end-proximal convoluted tubule perfusion site, early distal convoluted tubule collection site). As compared with the control period values, low-frequency (less than 1.0 Hz) renal nerve stimulation decreased absolute and fractional urinary flow rate and sodium and chloride excretion without affecting mean arterial pressure, glomerular filtration rate, renal blood flow, or renal vascular resistance. In the loop of Henle, the absorptive transport of water was not affected, whereas the absorptive transport of sodium and chloride was increased in both hydropenic (Na 111 +/- 49 peq/min) and isotonic saline volume-expanded rats (Na 154 +/- 69 peq/min, Cl 180 +/- 52 peq/min) during low-frequency renal nerve stimulation. Low-frequency renal nerve stimulation decreases urinary sodium and chloride excretion via a direct effect of increasing renal tubular sodium and chloride reabsorption. In addition to the established effect of increasing proximal tubular sodium reabsorption, Henle's loop sodium and chloride absorption are also increased, supporting a physiological role for the adrenergic innervation of these structures.
为评估肾神经刺激对亨利袢中钠、氯和水转运的影响，我们采用肾清除率和亨利袢微灌注技术（近端曲管灌注位点为末端，早期远端曲管收集位点），在麻醉状态下的禁水大鼠和等渗盐水扩容大鼠身上进行了实验。与对照期数值相比，低频（小于1.0赫兹）肾神经刺激降低了绝对和分数尿流率以及钠和氯排泄，而不影响平均动脉压、肾小球滤过率、肾血流量或肾血管阻力。在亨利袢中，水的吸收性转运未受影响，而在低频肾神经刺激期间，禁水大鼠（钠111±49皮摩尔/分钟）和等渗盐水扩容大鼠（钠154±69皮摩尔/分钟，氯180±52皮摩尔/分钟）的钠和氯吸收性转运均增加。低频肾神经刺激通过增加肾小管钠和氯重吸收的直接作用降低尿钠和氯排泄。除了增加近端肾小管钠重吸收这一既定作用外，亨利袢的钠和氯吸收也增加，支持了这些结构的肾上腺素能神经支配的生理作用。
