Luxon B A, King P D, Forker E L
Am J Physiol. 1982 Dec;243(6):G518-31. doi: 10.1152/ajpgi.1982.243.6.G518.
A digital computer is used to simulate solute disappearance curves evolving in the reservoir of a rat liver perfusion system. The results show that it is feasible to obtain good estimates of these kinetic parameters with only approximate information about the distribution of sinusoidal flows and the transfer function of the nonexchanging vasculature. By contrast satisfactory estimates of the transfer coefficients for uptake and efflux (but not excretion) are critically dependent on accurate measurements of the system transit times. A perfusion system suitable for implementing the method is tested and found to maintain normal liver function for upward of 60 min. The means to achieve the required volume and flow measurements are discussed, and an empirical criterion is developed for recognizing disappearance curves whose information content is too low to yield reliable results.
使用数字计算机模拟大鼠肝脏灌注系统储库中溶质消失曲线的演变。结果表明，仅利用关于正弦流分布和非交换血管系统传递函数的近似信息来获得这些动力学参数的良好估计是可行的。相比之下，摄取和流出（但不包括排泄）的传递系数的满意估计严重依赖于系统转运时间的精确测量。测试了一个适合实施该方法的灌注系统，发现其能在60分钟以上维持正常肝功能。讨论了实现所需体积和流量测量的方法，并制定了一个经验标准，用于识别信息含量过低而无法产生可靠结果的消失曲线。
