登革2型疫苗：在埃及伊蚊中的口服感染、传播及无证据表明病毒在蚊体内回复突变

Dengue-2 vaccine: oral infection, transmission, and lack of evidence for reversion in the mosquito, Aedes aegypti.

作者信息

Miller B R, Beaty B J, Aitken T H, Eckels K H, Russell P K

出版信息

Am J Trop Med Hyg. 1982 Nov;31(6):1232-7. doi: 10.4269/ajtmh.1982.31.1232.

DOI:10.4269/ajtmh.1982.31.1232

Abstract

The dengue-2 vaccine virus (S-1), and its parent virus (PR-159), were compared for their ability to infect orally, to replicate in, and subsequently to be transmitted by Aedes aegypti mosquitoes. The vaccine virus was markedly less efficient in its ability to infect mosquitoes orally. After ingesting infectious bloodmeals containing 3, 7 to 8.2 log10MID50/ml of the respective viruses, 56% (220/396) of the mosquitoes became orally infected with the parent virus contrasted with 16% (66/397) for the vaccine virus. None of the 16 infected mosquitoes transmitted the vaccine virus, while 14% (3/22) of the mosquitoes transmitted the parent virus. The vaccine virus remained temperature-sensitive (restrictive temperature 39 degrees C) after orally infecting and replicating in Ae. aegypti mosquitoes.

摘要

对登革2型疫苗病毒（S-1）及其亲代病毒（PR-159）在经口感染、在埃及伊蚊体内复制以及随后通过埃及伊蚊传播的能力方面进行了比较。疫苗病毒经口感染蚊子的能力明显较低。在摄入含有3、7至8.2 log10半数感染剂量（MID50）/毫升各自病毒的感染性血餐后，56%（220/396）的蚊子经口感染亲代病毒，相比之下，疫苗病毒感染率为16%（66/397）。16只感染的蚊子中没有一只传播疫苗病毒，而14%（3/22）的蚊子传播亲代病毒。在经口感染并在埃及伊蚊体内复制后，疫苗病毒仍对温度敏感（限制温度为39摄氏度）。