Troyer J M, Hanley K A, Whitehead S S, Strickman D, Karron R A, Durbin A P, Murphy B R
Department of Entomology, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA.
Am J Trop Med Hyg. 2001 Nov;65(5):414-9. doi: 10.4269/ajtmh.2001.65.414.
2Adelta30 is a live dengue-4 virus vaccine candidate with a 30-nucleotide deletion in its 3'-untranslated region. To assess the transmissibility of 2Adelta30 by mosquitoes, we compared its in vivo replication in mosquitoes with that of its wild type DEN-4 parent. Both the vaccine candidate and wild type virus were equally able to infect the mosquito Toxorhynchites splendens after intrathoracic inoculation. Relative to its wild type parent, 2Adelta30 was slightly restricted in its ability to infect the midgut of Aedes aegypti mosquitoes fed on an artificial blood meal and was even more restricted in its ability to disseminate from the midgut to the salivary glands. Thus, the 30-nucleotide deletion rendered the vaccine candidate more sensitive than its wild type parent to the mosquito midgut escape barrier. Most significantly, 2Adelta30 was not transmitted to 352 Ae. albopictus mosquitoes fed on 10 vaccinees, all of whom were infected with the vaccine candidate.
2Adelta30是一种登革4型活病毒候选疫苗，其3'非翻译区有30个核苷酸的缺失。为评估2Adelta30被蚊子传播的能力，我们将其在蚊子体内的复制情况与其野生型DEN - 4亲本进行了比较。候选疫苗和野生型病毒在胸腔内接种后均能同等程度地感染华丽巨蚊。相对于其野生型亲本，2Adelta30在感染以人工血餐为食的埃及伊蚊中肠的能力上略有受限，而从肠道扩散到唾液腺的能力受限更明显。因此，30个核苷酸的缺失使候选疫苗比其野生型亲本对蚊子中肠逃逸屏障更敏感。最显著的是，2Adelta30没有传播给以10名接种者为食的352只白纹伊蚊，所有这些接种者都感染了候选疫苗。
