Calisher C H, Kinney R M, de Souza Lopes O, Trent D W, Monath T P, Francy D B
Am J Trop Med Hyg. 1982 Nov;31(6):1260-72. doi: 10.4269/ajtmh.1982.31.1260.
Two strains of recently isolated Venezuelan equine encephalitis (VEE) complex virus from southern Brazil, avirulent for 6- to 8-week-old mice and short-haired guinea pigs, were characterized by biologic, serologic, and biochemical means. They were shown serologically to represent a single, newly recognized variant of subtype I. Two-dimensional polyacrylamide gel electrophoresis (PAGE) of ribonuclease T1 digests of viral ribonucleic acid showed considerable homology between the genomes of the new variant prototype and variant IA. Three structural proteins were visualized by discontinuous sodium dodecyl sulfate-PAGE (SDS-PAGE). Although the smallest protein of both recent isolates migrates with the capsid proteins of other subtype I viruses, the larger structural proteins of the new variants differ in molecular weight from the E1 and E2 envelope glycoproteins of the other subtype I variants. The new isolates produced peptide fragment patterns that were identical to each other, but different from the patterns of other subtype I viruses, following SDS-PaGE of dissociated virions digested with Staphylococcus aureus V8 protease. Since these two isolates were from Culex (Melanoconion) species mosquitoes and from a bat (Carollia perspicillata), were postulated that this is an enzootic VEE virus variant for which the classification IF is suggested.
从巴西南部最近分离出的两株委内瑞拉马脑炎（VEE）复合病毒，对6至8周龄小鼠和短毛豚鼠无毒力，通过生物学、血清学和生物化学方法进行了鉴定。血清学结果表明它们代表I型的一个新发现的单一变种。病毒核糖核酸的核糖核酸酶T1消化产物的二维聚丙烯酰胺凝胶电泳（PAGE）显示，新变种原型和变种IA的基因组之间有相当高的同源性。通过不连续十二烷基硫酸钠 - PAGE（SDS - PAGE）可观察到三种结构蛋白。尽管最近分离出的两株病毒中最小的蛋白与其他I型病毒的衣壳蛋白迁移情况相同，但新变种的较大结构蛋白在分子量上与其他I型变种的E1和E2包膜糖蛋白不同。在用金黄色葡萄球菌V8蛋白酶消化解离的病毒粒子进行SDS - PaGE后，新分离株产生的肽片段模式彼此相同，但与其他I型病毒的模式不同。由于这两株分离株来自库蚊（Melanoconion）属蚊子和一只蝙蝠（Carollia perspicillata），因此推测这是一种动物源性VEE病毒变种，并建议对其进行相应分类。
