Portaels F, Pattyn S R
Ann Microbiol (Paris). 1982 Sep-Oct;133(2):213-21.
The influence of pH on the growth of mycobacteria on Loewenstein and Dubos OAA media was studied with 56 strains belonging to 10 species of rapidly growing and 16 species of slowly growing mycobacteria. pH gradients were obtained by the use of McIlvaine's citrate and Sörensen's citrate buffer. The sensitivity of mycobacteria at different pH values of the medium was independent of the culture medium and buffer used. Minimal, maximal and optimal pH values were specific for each species, all strains of the same species producing identical results. Some slowly growing mycobacteria such as Mycobacterium lepraemurium developed within a very narrow optimal pH range: between 5.8 and 6.1. Other species developed over a wide optimal pH range, e.g. M. nonchromogenicum: from 5 to greater than 7.4. For slow growers, in general, with the exception of M. lepraemurium, optimal pH was between 5.8 and 6.5. In general, rapidly growing mycobacteria developed over a wider pH range of more than 2 units. All strains, with the exception of M. chelonei, developed optimally between pH 7 and 7.4. For M. chelonei, as for slower growing species, the optimal pH was between 5.4 and 6.5. Taxonomically related species such as M. tuberculosis and M. bovis, M. kansasii and M. gastri, M. microti and M. bovis BCG, and M. vaccae and M. parafortuitum, were inhibited or stimulated at identical pH values. This specificity constitutes a supplementary taxonomic character. At certain pH values, some species otherwise difficult to separate may be differentiated: e.g. M. avium, not growing at pH 4.6, is distinguished from M. scrofulaceum which does grow at pH 4.6. For routine diagnostic purposes, Loewenstein Jensen medium at pH 7 is not optimal; Ogawa medium, with its pH of 6, is more appropriate.
用属于10种快速生长型和16种缓慢生长型分枝杆菌的56株菌株，研究了pH对罗氏培养基和杜波斯OAA培养基上分枝杆菌生长的影响。通过使用麦克尔维恩柠檬酸盐和索伦森柠檬酸盐缓冲液获得pH梯度。培养基不同pH值下分枝杆菌的敏感性与所用培养基和缓冲液无关。每种菌株的最低、最高和最佳pH值都是特定的，同一菌株的所有菌株产生相同的结果。一些缓慢生长的分枝杆菌，如鼠麻风分枝杆菌，在非常窄的最佳pH范围内生长：5.8至6.1之间。其他菌株在较宽的最佳pH范围内生长，例如非产色分枝杆菌：从5至大于7.4。对于缓慢生长型菌株，一般来说，除鼠麻风分枝杆菌外，最佳pH在5.8至6.5之间。一般而言，快速生长型分枝杆菌在超过2个单位的更宽pH范围内生长。除龟分枝杆菌外，所有菌株在pH 7至7.4之间生长最佳。对于龟分枝杆菌，与生长较慢的菌株一样，最佳pH在5.4至6.5之间。分类学上相关的菌株，如结核分枝杆菌和牛分枝杆菌、堪萨斯分枝杆菌和胃分枝杆菌、田鼠分枝杆菌和卡介苗、母牛分枝杆菌和副偶然分枝杆菌，在相同pH值下受到抑制或刺激。这种特异性构成了一个补充的分类学特征。在某些pH值下，一些原本难以区分的菌株可能会被区分开来：例如，鸟分枝杆菌在pH值4.6时不生长，与在pH值4.6时生长的瘰疬分枝杆菌区分开来。出于常规诊断目的，pH值为7的罗氏培养基并非最佳选择；pH值为6的小川培养基更合适。
