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[使用水杨酸钠培养基培养的分枝杆菌培养物的鉴定]

[Identification of cultures of Mycobacteria cultivated using sodium salicylate medium].

作者信息

Lazovskaia A L, Pinchuk L M, Finkel'shteĭn L S, Fokina E I

出版信息

Probl Tuberk. 1994(5):45-6.

PMID:7870728
Abstract

Sodium salicylate test is used in bacteriological practice to distinguish M. tuberculosis and M. bovis from other mycobacterial species. The species of the strains growing in sodium salicylate media are not as a rule identified. This was done using gas chromatography with cultures which had grown in Lowenstein-Jensen media with sodium salicylate and isolated from the patients admitted to the Tuberculous hospital in 1991-1993. Only 20% of the strains belonged to human mycobacteria, the others were opportunistic or saprophyte mycobacteria (M. avium, M. fortuitum, M. phlei, M. flavescens, M. vaccae, M. smegmatis), some of the cultures were not of the Mycobacterium genus, but belonged to other Actinomycetales. It is evident that with sodium salicylate test one cannot be absolutely sure of Mycobacteria isolation. The strains growing in sodium salicylate medium call for further investigations.

摘要

水杨酸钠试验在细菌学实践中用于区分结核分枝杆菌和牛分枝杆菌与其他分枝杆菌种类。通常不会鉴定在水杨酸钠培养基中生长的菌株种类。这是通过气相色谱法对在含水杨酸钠的罗-琴培养基中生长并于1991 - 1993年从结核病医院收治的患者中分离出的培养物进行分析来完成的。只有20%的菌株属于人型分枝杆菌,其他的是机会性或腐生型分枝杆菌(鸟分枝杆菌、偶然分枝杆菌、草分枝杆菌、微黄分枝杆菌、母牛分枝杆菌、耻垢分枝杆菌),有些培养物不属于分枝杆菌属,而是属于其他放线菌目。显然,通过水杨酸钠试验不能绝对确定分枝杆菌的分离。在水杨酸钠培养基中生长的菌株需要进一步研究。

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