Zebrowska T, Simon O, Münchmeyer R, Wolf E, Bergner H, Zebrowska H
Arch Tierernahr. 1982 Jul;32(7-8):431-44. doi: 10.1080/17450398209435870.
Digesta were collected from 5 pigs of 33 kg live weight fitted with re-entrant cannulas in the duodenum (within 20-30 cm of the pylorus) and terminal ileum. The pigs received a diet of barley, soya bean oilmeal and a vitamin and mineral mixture. The flow rates of digesta, total nitrogen and the individual amino acids were measured at different time after feeding and during two 24 h periods. A marked increase in the flow of digesta, nitrogen and amino acids was seen in the duodenum after feeding. Total flow during 24 h of nitrogen and amino acids except His, Val, Leu, Phe and Met exceeded intake. Output of nitrogen and amino acids from the duodenal cannula was 117 and 108% of intake, respectively. A method to calculate the ratio of endogenous amino acids in digesta based on the amino acid composition of digesta, diet and endogenous secretions was developed. The calculated amounts of endogenous amino acids passing the proximal duodenum and terminal ileum were 32.2 and 21.9 g per 24 h, respectively. The greatest amount of endogenous amino acids passed through the duodenal cannula in the first two hours after feeding (2-3 g/h) and then gradually decreased to 1 g per hour. The results are discussed in relation to other studies on the secretion of endogenous protein and its amino acid composition.
从5头体重33千克、在十二指肠（幽门20 - 30厘米范围内）和回肠末端安装了再入式插管的猪收集食糜。这些猪采食大麦、豆粕以及维生素和矿物质混合物日粮。在喂食后的不同时间以及两个24小时期间测量了食糜、总氮和各个氨基酸的流速。喂食后十二指肠中食糜、氮和氨基酸的流速显著增加。24小时内除组氨酸、缬氨酸、亮氨酸、苯丙氨酸和蛋氨酸外的氮和氨基酸总流量超过摄入量。基于食糜、日粮和内源性分泌物的氨基酸组成，开发了一种计算食糜中内源性氨基酸比例的方法。通过十二指肠近端和回肠末端的内源性氨基酸计算量分别为每24小时32.2克和21.9克。喂食后的前两小时内通过十二指肠插管的内源性氨基酸量最大（2 - 3克/小时），然后逐渐降至每小时1克。结合其他关于内源性蛋白质分泌及其氨基酸组成的研究对结果进行了讨论。
