一种合成[32P]磷酸烯醇丙酮酸的简易酶法。
A simple enzymic method for the synthesis of [32P]phosphoenolpyruvate.
作者信息
Parra F
出版信息
Biochem J. 1982 Sep 1;205(3):643-5. doi: 10.1042/bj2050643.
Abstract
A rapid and simple enzymic method is described for the synthesis of [(32)P]phosphoenolpyruvate from [(32)P]P(i), with a reproducible yield of 74%. The final product was shown to be a good substrate for pyruvate kinase (EC 2.7.1.40).
摘要
描述了一种快速且简单的酶法，可从[(32)P]P(i)合成[(32)P]磷酸烯醇丙酮酸，产率可重复达到74%。最终产物被证明是丙酮酸激酶（EC 2.7.1.40）的良好底物。
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