一种用于制备规模合成腺苷 5'-[α-32P]三磷酸的简单酶法。
A simple enzymic method for the synthesis of adenosine 5'-[alpha-32P]triphosphate on a preparative scale.
作者信息
Martin B R, Voorheis H P
出版信息
Biochem J. 1977 Mar 1;161(3):555-9. doi: 10.1042/bj1610555.
Abstract
A simple, rapid and inexpensive method is described for the enzymic synthesis of [alpha-32P]ATP from [32P]Pi on a preparative scale with an overall yield of 53%. The final product contained all of the detectable radioactivity (less than 99.9%) in the alpha position and has been shown to behave identically with commerically availabe [alpha-32P]ATP during the synthesis of 3':5'-cyclic AMP in the reaction catalysed by adenylate cyclase.
摘要
本文描述了一种简单、快速且廉价的方法，可用于从[32P]Pi以制备规模酶促合成[α-32P]ATP，总产率为53%。最终产物在α位含有所有可检测到的放射性（小于99.9%），并且已证明在腺苷酸环化酶催化的反应中合成3':5'-环AMP时，其行为与市售的[α-32P]ATP相同。
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J Biol Chem. 1951 Dec;193(2):481-95.
2
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Biochem J. 1955 Sep;61(1):116-22. doi: 10.1042/bj0610116.
3
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Biochem J. 1964 Jan;90(1):147-9. doi: 10.1042/bj0900147.
4
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6
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