由α-氧代-γ-甲基硫代丁酸产生乙烯是黄孢原毛平革菌木质素分解活性的一种灵敏测定方法。
Ethylene production from alpha-oxo-gamma-methylthiobutyric acid is a sensitive measure of ligninolytic activity by Phanerochaete chrysosporium.
作者信息
Kelley R L, Reddy C A
出版信息
Biochem J. 1982 Aug 15;206(2):423-5. doi: 10.1042/bj2060423.
Abstract
Production of hydroxyl radical in ligninolytic cultures was determined by measuring the alpha-oxo-gamma-methylthiobutyric acid-dependent production of ethylene gas. The results showed that the pattern of ethylene production was very similar to that of ligninolytic activity [[14C]lignin leads to 14CO2). Furthermore, nutritional parameters, which are known to affect ligninolytic activity, affected OH.-radical-dependent ethylene production in a similar fashion. The results indicate that assay for ethylene production from alpha-oxo-gamma-methylthiobutyric acid is a simple and sensitive measure of ligninolytic activity by Phanerochaete chrysosporium.
摘要
通过测量α-氧代-γ-甲基硫代丁酸依赖的乙烯气体生成量来测定木质素分解培养物中羟基自由基的产生。结果表明，乙烯生成模式与木质素分解活性模式非常相似（[14C]木质素生成14CO2）。此外，已知影响木质素分解活性的营养参数，以类似方式影响羟基自由基依赖的乙烯生成。结果表明，测定α-氧代-γ-甲基硫代丁酸产生的乙烯是一种简单且灵敏的测量黄孢原毛平革菌木质素分解活性的方法。
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