Pulp and Paper Research Institute of Canada, 570 St. John's Boulevard, Pointe Claire, Quebec, Canada H9R 3J9.
Appl Environ Microbiol. 1992 Sep;58(9):3101-9. doi: 10.1128/aem.58.9.3101-3109.1992.
The discovery in 1983 of fungal lignin peroxidases able to catalyze the oxidation of nonphenolic aromatic lignin model compounds and release some CO(2) from lignin has been seen as a major advance in understanding how fungi degrade lignin. Recently, the fungus Trametes versicolor was shown to be capable of substantial decolorization and delignification of unbleached industrial kraft pulps over 2 to 5 days. The role, if any, of lignin peroxidase in this biobleaching was therefore examined. Several different assays indicated that T. versicolor can produce and secrete peroxidase proteins, but only under certain culture conditions. However, work employing a new lignin peroxidase inhibitor (metavanadate ions) and a new lignin peroxidase assay using the dye azure B indicated that secreted lignin peroxidases do not play a role in the T. versicolor pulp-bleaching system. Oxidative activity capable of degrading 2-keto-4-methiolbutyric acid (KMB) appeared unique to ligninolytic fungi and always accompanied pulp biobleaching.
1983 年发现真菌木质素过氧化物酶能够催化非酚类芳香族木质素模型化合物的氧化，并从木质素中释放出一些 CO(2)，这被认为是理解真菌如何降解木质素的重大进展。最近，发现彩绒革盖菌能够在 2 到 5 天内对未漂白纸浆进行实质性的脱色和脱木质素。因此，研究了木质素过氧化物酶在这种生物漂白中的作用。几种不同的测定表明，彩绒革盖菌可以产生和分泌过氧化物酶蛋白，但只有在某些培养条件下才会分泌。然而，使用新型木质素过氧化物酶抑制剂（偏钒酸盐离子）和使用染料azure B 的新型木质素过氧化物酶测定法的研究表明，分泌的木质素过氧化物酶在彩绒革盖菌纸浆漂白系统中不起作用。能够降解 2-酮-4-巯基丁酸（KMB）的氧化活性是木质素分解真菌所特有的，并且总是伴随着纸浆的生物漂白。
