Triadou P, Crepin M, Gros F, Lelong J C
Biochemistry. 1982 Nov 23;21(24):6060-5. doi: 10.1021/bi00267a006.
The synthesis and DNA binding activity of purified nuclear non-histone proteins from mouse erythroblasts and myoblasts have been compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, affinity chromatography, and protein blotting. The labeled non-histone proteins bound to mouse total DNA clearly differ between erythroid and muscle cell lines, but these differences mainly reflect the qualitative changes observed in their pattern of synthesis. By contrast, a cloned genomic mouse beta-globin DNA fragment binds specifically several proteins (100K, 65K, 50K, 45K, and 34K) from erythropoietic Friend cells and does not bind any protein in the corresponding fraction from myoblasts. The specificity of these DNA protein interactions requires a NaCl concentration of 0.1 M and a low protein/DNA ratio. In these conditions lambda DNA binds the above proteins to only a small extent. During the dimethyl sulfoxide induced terminal differentiation of Friend mouse erythroleukemia (MEL) cells, there is an apparent overall decrease of total as well as globin DNA binding to the nuclear non-histone proteins but not to the histones, whereas no significant qualitative changes are detected.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、亲和层析和蛋白质印迹法，对来自小鼠成红细胞和成肌细胞的纯化核非组蛋白的合成及DNA结合活性进行了比较。与小鼠总DNA结合的标记非组蛋白在红系和肌肉细胞系之间明显不同，但这些差异主要反映了其合成模式中观察到的定性变化。相比之下，克隆的基因组小鼠β-珠蛋白DNA片段特异性结合来自促红细胞生成性Friend细胞的几种蛋白质（100K、65K、50K、45K和34K），而成肌细胞相应部分未结合任何蛋白质。这些DNA-蛋白质相互作用的特异性需要0.1M的NaCl浓度和低蛋白/DNA比例。在这些条件下，λDNA仅与上述蛋白质有少量结合。在二甲基亚砜诱导的Friend小鼠红白血病（MEL）细胞终末分化过程中，与核非组蛋白而非组蛋白结合的总DNA以及珠蛋白DNA明显整体减少，而未检测到明显的定性变化。
