Curstedt T
Biochim Biophys Acta. 1982 Dec 13;713(3):589-601. doi: 10.1016/0005-2760(82)90319-8.
Rats were injected with [1,1-2H2]ethanol once every hour for 3-48 h and the fatty acid composition of and deuterium incorporation into different classes of hepatic phosphatidylcholines, phosphatidylethanolamines, phosphatidylserines and phosphatidylinositols were determined. Phosphatidylcholines and phosphatidylethanolamines with an oleoyl or a docosahexaenoyl residue at C-2 increased, while many of the species with a linoleoyl or an arachidonoyl residue at C-2 decreased throughout the experiment. No changes were seen in the phosphatidylinositols. The distribution of deuterium between the different positions of the glycerol moiety of the glycerophosphatides was similar for all species studied. The deuterium excess in molecules synthesized de novo was also similar for all species and remained relatively constant throughout the experiment. The mean excess was 8 atom% at C-1, 36 atom% at C-2 and 22 atom% at C-3 of the glycerol moiety. The apparent half-life times calculated for the glycerol moieties were different for the individual molecular species and varied between 1.1 and 36 h. However, the observed half-life times for the corresponding species of the different glycerophosphatides usually were similar. The deuterium excess in hepatic sn-glycerol 3-phosphate synthesized de novo was 8 atom% at C-1, 28 atom% at C-2 and 13 atom% at C-3. The excess at C-2 and C-3 was lower than that of the glycerol moiety of glycerophosphatides, indicating that a specific pool of sn-glycerol 3-phosphate with a gluconeogenetic origin was used for the biosynthesis of the different glycerophosphatides, and that the formation of this pool was closely coupled to ethanol oxidation. The deuterium excess in sn-glycerol moiety of the phosphatidylcholines indicated that these molecules had long half-life times or that the labelling of their precursor increased throughout the experiment.
大鼠每隔一小时注射一次[1,1-2H2]乙醇，持续3 - 48小时，然后测定不同种类的肝磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰丝氨酸和磷脂酰肌醇的脂肪酸组成以及氘掺入情况。在整个实验过程中，C-2位带有油酰基或二十二碳六烯酰基残基的磷脂酰胆碱和磷脂酰乙醇胺增加，而许多C-2位带有亚油酰基或花生四烯酰基残基的种类减少。磷脂酰肌醇未见变化。在所研究的所有种类中，甘油磷脂甘油部分不同位置之间的氘分布相似。所有种类从头合成分子中的氘过量情况也相似，并且在整个实验过程中保持相对恒定。甘油部分C-1位的平均过量为8原子%，C-2位为36原子%，C-3位为22原子%。为甘油部分计算的表观半衰期对于各个分子种类不同，在1.1至36小时之间变化。然而，不同甘油磷脂相应种类观察到的半衰期通常相似。从头合成的肝sn-甘油3-磷酸中，C-1位的氘过量为8原子%，C-2位为28原子%，C-3位为13原子%。C-2位和C-3位的过量低于甘油磷脂甘油部分的过量，表明具有糖异生来源的特定sn-甘油3-磷酸池用于不同甘油磷脂的生物合成，并且该池的形成与乙醇氧化密切相关。磷脂酰胆碱sn-甘油部分的氘过量表明这些分子具有长半衰期，或者其前体的标记在整个实验过程中增加。
