Fujita S, Peisach J
Biochim Biophys Acta. 1982 Nov 24;719(2):178-89. doi: 10.1016/0304-4165(82)90087-3.
The reduction of the azo dye, amaranth, by rat liver microsomes is inhibited about 90% by carbon monoxide, suggesting that the reaction largely depends on cytochrome P-450. Reducing equivalents for this reaction are supplied by NADPH. This reaction is stimulated by riboflavin, FMN and FAD, as well as by methylviologen. A large fraction of the stimulated reaction is not blocked by CO, indicating that there is a pathway of electron transfer which is independent of cytochrome P-450. Rat liver microsomes can reduce FAD, with reducing equivalents supplied by NADPH. The FADH2 thus produced is quickly oxidized by amaranth so that two FADH2 are oxidized for every amaranth reduced. The same stoichiometry is observed with photochemically prepared FADH2, formed in the absence of microsomes.
大鼠肝脏微粒体对偶氮染料苋菜红的还原作用被一氧化碳抑制约90%，这表明该反应很大程度上依赖于细胞色素P - 450。此反应的还原当量由NADPH提供。该反应受到核黄素、FMN和FAD以及甲基紫精的刺激。大部分受刺激的反应不受CO阻断，这表明存在一条独立于细胞色素P - 450的电子传递途径。大鼠肝脏微粒体可以还原FAD，其还原当量由NADPH提供。由此产生的FADH2会迅速被苋菜红氧化，因此每还原一个苋菜红会氧化两个FADH2。在无微粒体情况下光化学制备的FADH2也观察到相同的化学计量关系。
