Ramakrishnan S, Eagle M R, Houston L L
Biochim Biophys Acta. 1982 Nov 24;719(2):341-8. doi: 10.1016/0304-4165(82)90108-8.
A radioimmunoassay for ricin and ricin A- and B-chains was developed. Amounts as low as 100 pg of A-chain and 500 pg of B-chain could easily be quantitated. We showed, however, that the free chains were more reactive in the radioimmunoassay than the equivalent quantity of the individual chains when combined in intact ricin. The usefulness of the assay was demonstrated by determining the concentration of contaminating A- or B-chains in preparations of the separate polypeptides purified by DEAE Bio-Gel A chromatography and by chromatofocusing.
开发了一种用于检测蓖麻毒素及其A链和B链的放射免疫分析法。低至100 pg的A链和500 pg的B链量都能轻松定量。然而，我们发现，在放射免疫分析中，游离链比完整蓖麻毒素中同等量的单个链组合时更具反应性。通过测定经DEAE生物凝胶A色谱法和色谱聚焦法纯化的单独多肽制剂中污染的A链或B链浓度，证明了该分析方法的实用性。
