White-Scharf M E, Imanishi-Kari T
Eur J Immunol. 1982 Nov;12(11):935-42. doi: 10.1002/eji.1830121108.
A comparative antigenic analysis was carried out to determine whether cross-reactivity exists between the major idiotypic responses to (4-hydroxy-3-nitrophenyl)acetyl (NP) in BALB/c and C57BL/6 mice. Extensive cross-reactivity exists between the NPa (BALB/c) and NPb (C57BL/6) allotype-linked idiotypic responses to NP. The cross-reactive determinants of the NPb idiotype are confined to one particular group of NPb-positive monoclonal antibodies. The extent of cross-reactivity between this group of C57BL/6 antibodies and idiotype-positive monoclonal antibodies of BALB/c is so great that they cannot be readily distinguished as NPb- or NPa-positive antibodies with polyclonal anti-idiotypic reagents. That this cross-reactivity is not unique to monoclonal antibodies was confirmed by the demonstration of these cross-reactive determinants in the immunesera of individual BALB/c and C57BL/6 mice. Additionally, evidence was obtained from these experiments and from earlier ones from this laboratory which suggests that the BALB/c idiotypic response to NP-protein conjugate is more homogeneous than the C57BL/6 idiotypic responses.
进行了一项比较抗原分析，以确定BALB/c和C57BL/6小鼠对（4-羟基-3-硝基苯基）乙酰基（NP）的主要独特型反应之间是否存在交叉反应。NPa（BALB/c）和NPb（C57BL/6）与同种异型相关的针对NP的独特型反应之间存在广泛的交叉反应。NPb独特型的交叉反应决定簇局限于一组特定的NPb阳性单克隆抗体。这组C57BL/6抗体与BALB/c独特型阳性单克隆抗体之间的交叉反应程度非常高，以至于用多克隆抗独特型试剂无法轻易将它们区分为NPb或NPa阳性抗体。通过在个体BALB/c和C57BL/6小鼠的免疫血清中证明这些交叉反应决定簇，证实了这种交叉反应并非单克隆抗体所特有。此外，从这些实验以及本实验室早期的实验中获得的证据表明，BALB/c对NP-蛋白质偶联物的独特型反应比C57BL/6独特型反应更具同质性。
