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Structural domains of ribosomal protein S8 and their relationship to ribosomal RNA binding.

作者信息

Paterakis K, Littlechild J

出版信息

FEBS Lett. 1982 Nov 29;149(2):328-33. doi: 10.1016/0014-5793(82)81125-3.

DOI:10.1016/0014-5793(82)81125-3
PMID:7152046
Abstract

Escherichia coli ribosomal protein S8 has been subjected to mild proteolytic digestion in order to search for structural domains within the protein [1]. A characteristic fragment produced in high yield after chymotrypsin treatment has been located with the protein sequence. Circular dichroism has shown this domain to be rich in alpha helix. However, the fragment loses its ability to bind to 16S rRNA as does a similar fragment produced by trypsin cleavage. The intact protein is required for rRNA binding and is highly protected against proteolytic digestion when bound to the RNA.

摘要

相似文献

1
Structural domains of ribosomal protein S8 and their relationship to ribosomal RNA binding.
FEBS Lett. 1982 Nov 29;149(2):328-33. doi: 10.1016/0014-5793(82)81125-3.
2
Interaction of Escherichia coli ribosomal protein S8 with its binding sites in ribosomal RNA and messenger RNA.大肠杆菌核糖体蛋白S8与其在核糖体RNA和信使RNA中的结合位点的相互作用。
J Mol Biol. 1988 Nov 20;204(2):295-307. doi: 10.1016/0022-2836(88)90577-3.
3
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Nucleic Acids Res. 1975 Jun;2(6):931-41. doi: 10.1093/nar/2.6.931.
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Secondary structure of a 345-base RNA fragment covering the S8/S15 protein binding domain of Escherichia coli 16S ribosomal RNA.覆盖大肠杆菌16S核糖体RNA的S8/S15蛋白结合结构域的345个碱基的RNA片段的二级结构。
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Ribosomal protein-nucleic acid interactions. I. Isolation of a polypeptide fragment from 30S protein S8 which binds to 16S rRNA.核糖体蛋白与核酸的相互作用。I. 从与16S rRNA结合的30S蛋白S8中分离出一个多肽片段。
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Binding of Escherichia coli ribosomal protein S8 to 16 S rRNA. A model for the interaction and the tertiary structure of the RNA binding site.大肠杆菌核糖体蛋白S8与16S rRNA的结合。RNA结合位点相互作用及三级结构模型。
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7
Protein binding sites on Escherichia coli 16S ribosomal RNA; RNA regions that are protected by proteins S7, S9 and S19, and by proteins S8, S15 and S17.大肠杆菌16S核糖体RNA上的蛋白质结合位点；受蛋白质S7、S9和S19以及蛋白质S8、S15和S17保护的RNA区域。
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Binding sites for ribosomal proteins S8 and S15 in the 16 S RNA of Escherichia coli.大肠杆菌16S RNA中核糖体蛋白S8和S15的结合位点
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Eur J Biochem. 1994 Jul 15;223(2):437-45. doi: 10.1111/j.1432-1033.1994.tb19011.x.

引用本文的文献

1
Mutagenesis of ribosomal protein S8 from Escherichia coli: defects in regulation of the spc operon.大肠杆菌核糖体蛋白S8的诱变：spc操纵子调控缺陷
J Bacteriol. 1992 Feb;174(4):1213-21. doi: 10.1128/jb.174.4.1213-1221.1992.