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一段核糖体蛋白S4片段的特性描述，该片段受到大肠杆菌16S核糖体RNA的保护而免受胰蛋白酶消化。

The characterisation of a fragment of ribosomal protein S4 that is protected against trypsin digestion by 16S ribosomal RNA of Escherichia coli.

作者信息

Schulte C, Schiltz E, Garrent R

出版信息

Nucleic Acids Res. 1975 Jun;2(6):931-41. doi: 10.1093/nar/2.6.931.

DOI:10.1093/nar/2.6.931
PMID:1096086
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343479/
Abstract

After mild trypsin treatment of a complex of ribosomal protein S4 and 16S RNA of Escherichia coli, a large homogeneous fragment of the S4 protein was protected against digestion by its RNA binding site. This fragment was isolated and characterised for molecular weight. It was able to rebind specifically to 16S RNA. Preliminary results indicate that protected protein fragments can also be obtained from other proteins that complex specifically with 23S and 5S RNA.

摘要

用温和的胰蛋白酶处理大肠杆菌核糖体蛋白S4与16S RNA的复合物后，S4蛋白的一个大的均一性片段因其RNA结合位点而免受消化。分离出该片段并对其分子量进行了表征。它能够特异性地重新结合到16S RNA上。初步结果表明，受保护的蛋白片段也可以从与23S和5S RNA特异性结合的其他蛋白中获得。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48ed/343479/650f0d3653ba/nar00503-0175-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48ed/343479/650f0d3653ba/nar00503-0175-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48ed/343479/650f0d3653ba/nar00503-0175-a.jpg

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本文引用的文献

1
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
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Radioactive labeling of proteins in vitro.蛋白质的体外放射性标记
J Biol Chem. 1971 Feb 10;246(3):831-2.
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Ribosomal proteins. Isolation of the proteins from 30S ribosomal subunits of Escherichia coli.核糖体蛋白。从大肠杆菌30S核糖体亚基中分离蛋白质。
大肠杆菌50S核糖体亚基中蛋白质对抗体的可及性：一项超速离心研究。
Mol Gen Genet. 1977 Mar 16;151(3):245-52. doi: 10.1007/BF00268787.
Eur J Biochem. 1971 Nov 11;23(1):7-11. doi: 10.1111/j.1432-1033.1971.tb01584.x.
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Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.核糖体蛋白。VII. 用于核糖体蛋白指纹图谱分析的二维聚丙烯酰胺凝胶电泳
Anal Biochem. 1970 Aug;36(2):401-12. doi: 10.1016/0003-2697(70)90376-3.
5
Assembly mapping of 30S ribosomal proteins from E. coli.来自大肠杆菌的30S核糖体蛋白的装配图谱
Nature. 1970 Jun 27;226(5252):1214. doi: 10.1038/2261214a0.
6
The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins.大肠杆菌的核糖体蛋白。I. 30S核糖体蛋白的纯化。
Biochemistry. 1969 Jul;8(7):2897-905. doi: 10.1021/bi00835a031.
7
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Eur J Biochem. 1974 Oct 2;48(2):513-8. doi: 10.1111/j.1432-1033.1974.tb03792.x.
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Can J Biochem. 1974 Nov;52(11):1038-43. doi: 10.1139/o74-145.
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