Atsumi T, Shirayoshi Y, Takeichi M, Okada T S
Differentiation. 1982;23(1):83-6. doi: 10.1111/j.1432-0436.1982.tb01269.x.
By fusion of nullipotent embryonal carcinoma F9 cells with certain somatic cells freshly collected from differentiated tissues such as thymus and lens, pluripotent hybrid cell lines were obtained. They exhibited a wide spectrum of differentiation, including neural tubes, cartilages, skeletal muscles, ciliated epithelia and others, in solid tumors formed after injection into syngeneic mice. Cells from these tumors differentiated into several cell types when cultured in vitro. A possibility of the introduction of genes to code the factors for regulating differentiation into F9 cells by fusion is suggested.
通过将无能的胚胎癌细胞F9与从分化组织（如胸腺和晶状体）新鲜收集的某些体细胞融合，获得了多能杂交细胞系。将它们注射到同基因小鼠体内后形成的实体瘤中，它们表现出广泛的分化，包括神经管、软骨、骨骼肌、纤毛上皮等。当在体外培养时，这些肿瘤的细胞分化成几种细胞类型。这表明通过融合将编码调节分化因子的基因引入F9细胞具有可能性。
